Compositional homogeneity tests¶

The usual, poor, way to do a compositional homogeneity test is to do a Chi-square test on the data. Paup does this on the data overall, and Tree-Puzzle does it on individual sequences. In the latter, since there are many simultaneous comparisons, the power of the conclusions might be compromized. P4 does the test both ways, using the method Data.Data.compoChiSquaredTest().

This test uses the X^2 statistic. It is well-known that this sort of test suffers from a high probability of type II error, because the chi-square curve is not an appropriate null distribution by which to assess significance of the X^2 statistic.

A better null distribution can be obtained by simulating data on the tree and model in question, and using X^2 statistics extracted from those simulations to make a null distribution appropriate to the problem at hand. This is done using the Tree method Tree.Tree.compoTestUsingSimulations().

Model fit tests¶

For Bayesian model fit assessment you can do posterior predictive simulations during the MCMC. See Posterior predictive simulations.

P4 implements two ML-based model fit tests– the tree- and model-based composition fit test, and Goldman’s version of the Cox test. Both rely on simulations that generally need to be followed by optimizations, so they are expensive tests. In both tests the simulation/optimization part is the time-consuming part, and since both tests can use the same simulations, in p4 they are done together. First, the simulations are done using the Tree method Tree.Tree.simsForModelFitTests(). After that, the simulation results are digested with the Tree method Tree.Tree.modelFitTests().